The analysis of free urinary Cortisol (CFU) constitutes the first approach for the screening of endogenous Cushing syndrome (CS) at biochemical laboratory level. CFU measured within 24 hours can be useful also in other clinical conditions characterized by a high level of Cortisol in serum, for example apparent mineralocorticoid excess syndrome (AME).
Cortisol is mainly secreted by adrenal glands, while Cortisone is mainly produced by 11ß-hydroxysteroid dehydrogenase 2 that converts bioactive Cortisol in inactive Cortisone preventing the activation of mineralocorticoid receptor caused by Cortisol. Cortisol and Cortisone simultaneous determination is very important for the diagnosis of AME, CS but also congenital adrenal hyperplasia and adrenal insufficiency.
EUM06200_FloMass@ Urinary Free Cortisol and Cortison in Serum by LC-MS/MS