Features
Type of PCR
Quantitative real-time PCR (qPCR)
Description
The AquaScreen® Legionella species qPCR Detection Kit is used for DNA samples prepared with the AquaScreen® Fast Extract kit for quantification of Legionella in waters samples. The supplied primer set is specific for a broad range of legionella species, but does not detect other waterborne bacteria as required by ISO/TS 12869:2012.
Scope of Application
Applicable for water testing as described in ISO/TS 12869:2012, in research and industry for QA testing of process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.
Kit Components
Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water
Package Sizes
Cat. No. 33-2025 25 reactions
Cat. No. 33-2100 100 reactions
Cat. No. 33-2250 250 reactions
Required Consumables
PCR reaction tubes
Optional: For quantification we recommend our Legionella pneumophila PCR Quantification Standard (Cat. No. 52-0101).
Required Lab Equipment
qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips
Storage
The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below -18 °C.
Compliance
AFNOR XP T90-471 and ISO/TS 12869:2012
