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ortho-Cresol and Phenol in Urine
Complete Kit, Order No. 53 000

 

o-Cresol is a minor metabolite of toluene and serves for the assessment of an exposure to toluene, which is a frequently used solvent in glues, paints and thinners. In contrast to hippuric acid (see order no. 52000), which is the main metabolite of toluene, o-cresol is no physiological component of urine and therefore reflects the internal exposure towards the polllutant.Phenol is the main metabolite of benzene and is used for the assessment to benzene as well as to phenol itself.

The ClinTox® complete kit of RECIPE provides an excellent analytical sensitivity and enables a reliable biomonitoring of both analytes. The sample clean up is performed via solid phase extraction (SPE).

 
  • Sample clean-up via SPE


  • High analytical sensitivity


  • Injection interval: 15 min


Test Data

  

HPLC Parameters
Linearity: o-Cresol: 0.05 - 50 mg/l
  Phenol: 0.2 - 50 mg/l
Recovery: 85 - 95 %
Lower detection limit: o-Cresol: 0.03 mg/l
  Phenol: 0.1 mg/l
Lower determination limit: o-Cresol: 0.05 mg/l
  Phenol: 0.2 mg/l
Intraassay precision: o-Cresol: 2.4 %
  Phenol: 3.2 %
Interassay precision: o-Cresol: 2.8 %
  Phenol: 4.8 %
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 10 - 20 µl
Injection interval: 15 min
Fluorescence detector: 277 nm (exc.), 300 nm (em.)
HPLC-Thermostat: 30°C

 

Sample Preparation

53000_flow

 

Chromatograms
ClinChek® Urine Control, Level I ClinCheck® Urine Control, Level II
53000_chrom1   53000_chrom2
Phenol: 5.62 min, p-Cresol: 9.87 min, o-Cresol: 11.06, IS: 12.39 min   Phenol: 5.62 min, p-Cresol: 9.87 min, o-Cresol: 11.07, IS: 12.39 min

 

ortho-Cresol and Phenol in Urine
Complete Kit, Order No. 53 000

Ordering Information

Order No. Description Quantity
53000  ClinTox® Complete Kit for o-Cresol and Phenol in Urine for 100 assays 1 pce. 
 
  Kit Components (order no. 53000):  
53010 Mobile Phase 1000 ml
53012 IS Internal Standard 5 ml
9969 Urine Calibrator, lyophil. 5 x 10 ml
52320 Sample Preparation Columns 50 pcs
within the kit: 2 x order no. 53020
53021 Reagent A 400 ml
53022 Reagent B 300 ml
within the kit: 2 x order no. 53022
53023 Reagent C 25 ml
53024 Reagent D 2 ml
within the kit: 3 x order no. 53024
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
52330 Analytical Column with test chromatogram 1 pce.
 
  Accessories:  
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls for toxic organic compounds:  
8867 Urine Control, lyophil., Level I 10 x 10 ml
8868 Urine Control, lyophil., Level II 10 x 10 ml
8869 Urine Control, lyophil., Level I, II 2 x 5 x 10 ml

 

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