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Hippuric Acid and Methylhippuric Acids in Urine
Complete Kit, Order No. 52 000

 

The ClinTox® complete kit of RECIPE is designed for the reliable biomonitoring of hippuric acid and of o-, m- and p-methylhippuric acid. Those compounds are the urinary metabolites of toluene and of the corresponding xylenes and thus reflect an exposure level towards these pollutants.

The sample preparation column, the analytical column, and the reference materials of this kit can be also used for the determination of o-cresol and phenol in urine (see order no. 53000).

  
  • Sample clean-up via SPE

  • High analytical sensitivity

  • Injection interval: 8 min


Test Data

  

HPLC Parameters
Linearity: Hippuric Acid:
  50 - 10000 mg/l
  Methylhippuric Acids:
  5 - 5000 mg/l
Recovery: 85 - 95 %
Lower detection limit: Hippuric Acid: 30 mg/l
  Methylhip. Acids: 2 mg/l
Lower determination limit: Hippuric Acid: 50 mg/l
  Methylhip. Acids: 5 mg/l
Intraassay precision: Hippuric Acid: 2.0 %
  Methylhip. Acids: 2.1 %
Interassay precision: Hippuric Acid: 2.3 %
  Methylhip. Acids: 3.2 %
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 20 µl
Injection interval: 8 min
UV-Detector: 230 nm
HPLC-Thermostat: 30°C

 

Sample Preparation

52000_flow

 

Chromatograms
ClinChek® Urine Control, Level I ClinCheck® Urine Control, Level II
52000_chrom1   52000_chrom2
Hippuric acid: 3.19 min, o-Methylhippuric acid: 4.23 min, IS: 5.07 min, m-/p-Methylhippuric acid: 6.43 min   Hippuric acid: 3.24 min, o-Methylhippuric acid: 4.29 min, IS: 5.14 min, m-/p-Methylhippuric acid: 6.52 min

 

Hippuric Acid and Methylhippuric Acids in Urine
Complete Kit, Order No. 52 000

Ordering Information

Order No. Description Quantity
52000  ClinTox® Complete Kit for Hippuric Acid and Methylhippuric Acids in Urine for 100 assays 1 pce. 
 
  Kit Components (order no. 52000):  
52010 Mobile Phase 1000 ml
52011 Standard Solution 3 ml
52012 IS Internal Standard 10 ml
9969 Urine Calibrator, lyophil. 5 x 10 ml
52320 Sample Preparation Columns 50 pcs
within the kit: 2 x order no. 52320
52021 Reagent A 400 ml
52022 Reagent B 400 ml
within the kit: 2 x order no. 52022
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
52330 Analytical Column with test chromatogram 1 pce.
 
  Accessories:  
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls for toxic organic compounds:  
8867 Urine Control, lyophil., Level I 10 x 10 ml
8868 Urine Control, lyophil., Level II 10 x 10 ml
8869 Urine Control, lyophil., Level I, II 2 x 5 x 10 ml

 

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