Mycophenolic Acid in Plasma
Complete Kit, Order No. 29 000

 

Mycophenolate mofetil (MMF) belongs to the newer immunosuppressive drugs and is usually used in combination with cyclosporine A or tacrolimus. As a pro-drug, MMF is rapidly hydrolized in vivo to its active constituent mycophenolic acid (MPA). In order to reach an optimal therapeutic efficiency, i.e. to maximize drug efficiency while minimizing its toxicity, the administration of MMF requires a monitoring of the MPA concentration with subsequent dosage adjustment.

For this purpose, the ClinRep® HPLC complete kit of RECIPE provides an easy and quick sample preparation and enables a robust and reliable analysis. For quality assurance, RECIPE offers reliable calibration and control materials.

 
  • No time consuming solid phase extraction required


  • Quick and easy sample preparation





Test Data

 

HPLC Parameters

Linearity: 0.1 - 40 mg/l
Recovery: 95 - 105 %
Lower detection limit: 0.06 mg/l
Lower determination limit: 0.1 mg/l
Intraassay precision: 3.3 %
Interassay precision: 4.0 %
 
Pump: isocratic pump,
flow rate: 1.2 ml/min
Injection volume: 50 µl
Injection interval: 7 min
UV-detector: 215 nm
HPLC-Thermostat: 30°C

 

Sample Preparation

29000_flow

 

Chromatograms

ClinChek® Plasma Control, Level I ClinChek® Plasma Control, Level II
29000_chrom1   29000_chrom2
Mycophenolic acid: 4.99 min, IS: 6.05 min  
Mycophenolic acid: 4.99 min, IS: 6.05 min


Mycophenolic Acid in Plasma
Complete Kit, Order No. 29 000

Ordering Information

Order No. Description Quantity
29000  ClinRep® Complete Kit for Mycophenolic Acid in Plasma for 100 assays 1 pce. 
 
  Kit Components (order no. 29000):  
29010 Mobile Phase 1000 ml
29014 Test Solution 3 ml
29012 IS Internal Standard 2 ml
29013 Plasma Calibrator, lyophil. 3 ml
29020 Sample Preparation Vials 100 pcs.
29021 P Precipitant 20 ml
  Manual 1 pce.
  Quick Reference 1 pce.
     
  Start Accessories:  
29030 Analytical Column with test chromatogram 1 pce.
     
  Accessories:  
FK5810 PEEK-tubing (connection of prefilter and column 1 pce.
FK5820 Stainless steel prefiilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
29080 Plasma Control, lyophil., Level I 10 x 3 ml
29081 Plasma Control, lyophil., Level II 10 x 3 ml
29082 Plasma Control, lyophil., Level III 10 x 3 ml
29083 Plasma Control, lyophil., Level I, II, III 3 x 3 x 3 ml


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