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25-OH Vitamin D2 / D3 in Plasma and Serum with UPLC® Complete Kit, Order No. 35 100

 

25-OH-Vitamin D (i.e. D2, D3) is an important parameter for the assessment of the vitamin D status. In addition to RECIPE's HPLC complete kit (order no. 35000), a ClinRep® Complete Kit for WATERS UPLC® systems is available now.

The RECIPE ClinRep UPLC® Complete Kit features a significantly increased sample throughput with reduced buffer consumption due to the very short chromatography duration of only 2 minutes. Laboratories and patients benefit from the quality and reliability of a CE certified analytical method, as is the case with our HPLC test kit. For system check, calibration and internal quality control, we are offering a ClinTest® Test Solution, a ClinCal® Serum Calibrator and ClinChek® Serum Controls. The ClinCal® Serum Calibrator is traceable to a certified reference material.

 
  • New Testkit for UPLC®

  • Quick and easy sample preparation
    (protein precipitation)


  • Injection interval: 2 min.


  • Reference materials with high stability available


Test Data UPLC Parameters Linearity: 4.6 - 500 µg/l (25-OH-Vit. D2) 3.7 - 500 µg/l (25-OH-Vit. D3) Recovery: 99 - 104 % Lower detection limit: 2.8 µg/l (25-OH-Vit. D2) 2.2 µg/l (25-OH-Vit. D3) Lower determination limit: 4.6 µg/l (25-OH-Vit. D2) 3.7 µg/l (25-OH-Vit. D3) Intraassay precision: 4.3 % (25-OH-Vit. D2) 4.6 % (25-OH-Vit. D3) Interassay precision: 5.9 % (25-OH-Vit. D2) 3.9 % (25-OH-Vit. D3) Pump: isocratic pump, flow rate: 0.75 ml/min Injection volume: 10 µl Injection interval: 2 min UV detector: 264 nm Column heater: 40 °C

Test Data

 

UPLC Parameters
Linearity: 4.6 - 500 µg/l (25-OH-Vit. D2)
  3.7 - 500 µg/l (25-OH-Vit. D3)
Recovery: 99 - 104 %
Lower detection limit: 2.8 µg/l (25-OH-Vit. D2)
  2.2 µg/l (25-OH-Vit. D3)
Lower determination limit: 4.6 µg/l (25-OH-Vit. D2)
  3.7 µg/l (25-OH-Vit. D3)
Intraassay precision: 4.3 % (25-OH-Vit. D2)
  4.6 % (25-OH-Vit. D3)
Interassay precision: 5.9 % (25-OH-Vit. D2)
  3.9 % (25-OH-Vit. D3)
 
Pump: isocratic pump, flow rate: 0.75 ml/min
Injection volume: 10 µl
Injection interval: 2 min
UV detector: 264 nm
Column heater: 40 °C

 

Sample Preparation

25-OH Vitamin D2 / D3 in Plasma and Serum with UPLC®

 

Chromatograms

ClinChek® Serum Control, Level I
25-OH Vitamin D3: 0.68min, 25-OH Vitamin D2: 0.77, IS: 1.58 min 		ClinCheck® Serum Control, Level II
25-OH Vitamin D3: 0.68 min, 25-OH Vitamin D2: 0.77, IS: 1.57 min
35100_Level I 35100

 

 

25-OH Vitamin D2 / D3 in Plasma and Serum with UPLC® Complete Kit, Order No. 35 100

Ordering Information



Order No. Description Quantity
35100  ClinRep® Complete Kit for 25-OH Vitamin D2 / D3 in Plasma and Serum with UPLC®
for 100 assays		 1 pce. 
 
  Kit Components (order no. 35100):  
35010 Mobile Phase 1000 ml
35012 Internal Standard IS 50 ml
35013 Serum Calibrator, lyophil. 5 x 2 ml
35014 Test Solution, lyophil. 2 x 2 ml
35020 Sample Preparation Vials 100 pcs.
35021 P Precipitant 50 ml
35120 Diluting Vials 100 pcs.
35122 D Diluting Solution 20 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
35130 Analytical Column with test chromatogram 1 pce.
 
 
  ClinChek® Controls:  
35080 Serum Control, lyophil., Level I 10 x 2 ml
35081 Serum Control, lyophil., Level II 10 x 2 ml
35082 Serum Control, lyophil., Level I, II 2 x 5 x 2 ml


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