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Homocysteine in Plasma
Complete Kit, Order No. 23000

 

Homocysteine is an established marker to estimate the risk towars atherosclerosis and coronary heart disease. An elevated plasma homocysteine level (hyperhomocysteinemia), whether due to nutrient deficiencies or by genetic defects, can be reduced by intake of folic acid, vitamin B6 and B12. Therefore, the determination of homocysteine does not only serve for the risk assessment towards atherosclerotic affections but also to ensure the proper combination and dosage of a nutrition supplement therapy.

The test kit of RECIPE allows the simultaneous analysis of total homocysteine (tHcys) and total cysteine (tCys). The analytical method features in a reliable sample preparation (specific derivatisation reagent) and a quick chromatography (injection interval: 5 min).

  
  • Determination of tHcys and tCys


  • Reliable sample preparation
    (specific derivatisation
    of the analytes)


  • Quick chromatography:
    ( Injection interval: 5 min)


Test Data

  

HPLC Parameters
Linearity: 1 - 100 µmol/l
Recovery: 95 - 105 %
Lower detection limit: 0.5 µmol/l
Lower determination limit: 1 µmol/l
Intraassay precision: 3.9 %
Interassay precision: 5.8 %
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 20 µl
Injection interval: 5 min
Fluorescence detector: 385 nm (exc.), 515 (em.)
HPLC-Thermostat: 30°C

 

Sample Preparation

23000_flow

 

Chromatograms
ClinChek® Plasma Control, Level I ClinCheck® Plasma Control, Level II
23000_chrom1   23000_chrom2
Cysteine: 2.10 min, IS: 2.47 min, Cysteinylglycine: 2.85,
Homocysteine: 3.31 min		   Cysteine: 2.11 min, IS: 2.48 min, Cysteinylglycine: 2.85,
Homocysteine: 3.31 min


Homocysteine in Plasma
Complete Kit, Order No. 23000

Ordering Information

Order No. Description Quantity
23000  ClinRep® Complete Kit for Homocysteine in Plasma for 100 assays 1 pce. 
 
  Kit Components (order no. 23000):  
23010 Mobile Phase 1000 ml
23011 Standard Solution 3 ml
23012 IS Internal Standard 3 ml
23013 Plasma Calibrator, lyophil. 3 ml
23020 Sample Preparation Vials 100 pcs.
23021 Reagent A 3 ml
23022 Reagent B 10 ml
23023 Reagent C 10 ml
23024 Reagent D 5 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
23030 Analytical Column with test chromatogram 1 pce.
 
  Accessories:  
23060 250 µl derivatisation vial with cap (PE) 50 pcs.
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
23080 Plasma Control, lyophil., Level I 10 x 3 ml
23081 Plasma Control, lyophil., Level II 10 x 3 ml
23082 Plasma Control, lyophil., Level I, II 2 x 5 x 3 ml


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