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Porphyrins Differentiated in Urine
Complete Kit, Order No. 17 000

 

Metabolic diseases that lead to an accumulation of porphyrins in blood and tissues are summarized in the term "porphyrias". The particular type of porphyria is diagnosed by the investigation of the specific porphyrin excretion profile.

For this purpose, the ClinRep® complete kit of RECIPE allows to separate (differentiate) the diverse porphyrins with high resolution (in different porhyrin-isomers!) and enables an exact quantification. As quick tests for the detection of porphyrias a column test for the determination of the total porphyrins (see order no. 17100) as well as a column test for the determination of 5-aminolevulinic acid and porphobilinogen are available (see order no. 17200).

  
  • High resolution of the porphyrin profile

  • Only 1 pretreatment step prior to sample injection

  • Reliable test results


Test Data

  

HPLC Parameters
Linearity: 1 - 5000 µg/l
  (per analyte)
Lower detection limit: 0.5 µg/l
  (per analyte)
Lower determination limit: 1 µg/l
  (per analyte)
Intraassay precision: 2.7 % (Uro)
  2.7 % (Hepta)
  3.3 % (Hexa)
  1.9 % (Penta)
  1.7 % (Copro I)
  1.6 % (Copro III)
Interassay precision: 3.4 % (Uro)
  2.3 % (Hepta)
  3.8 % (Hexa)
  2.2 % (Penta)
  1.8 % (Copro I)
Interassay precision: 1.9 % (Copro III)
 
Pump: binary gradient pump,
flow rate: 0.7 ml/min
Injection volume: 20 µl
Injection interval: 20 min
Fluorescence detector: 394 nm (exc.), 624 nm (em.)
HPLC-Thermostat: 30°C
   
Abbreviations:
Uro: Uroporphyrin
Hepta: Heptacarboxyporphyrin I
Hexa: Hexacarboxyporphyrine (Iac and Iab-Isomer)
Penta: Pentacarboxyporphyrin I
Copro: Coproporphyrin

 

Sample Pretreatment

sample preparation

 

Chromatograms
ClinChek® Urine Control, Level I ClinCheck® Urine Control, Level II
 
Uroporhyrin I: 5.19 min, Heptacarboxyporphyrin I: 6.31 min, Hexacarboxyporphyrins (Iac and Iab Isomer): 7.54 min, Internal Standard IS: 8.86 min, Pentacarboxyporphyrin I: 9.55 min, Coproporphyrin I: 11.39 min, Coproporphyrin III: 12.68 min.   Uroporhyrin I: 5.16 min, Heptacarboxyporphyrin I: 6.28 min, Hexacarboxyporphyrins (Iac and Iab Isomer): 7.53 min, Internal Standard IS: 8.86 min, Pentacarboxyporphyrin I: 9.55 min, Coproporphyrin I: 11.37 min, Coproporphyrin III: 12.67 min.


Porphyrins Differentiated in Urine
Complete Kit, Order No. 17 000

Ordering Information

Order No. Description Quantity
17000  ClinRep® Complete Kit for Porphyrins Differentiated in Urine for 100 assays 1 pce. 
 
  Kit Components (order no. 17000):  
17008 Mobile Phase A 1000 ml
within the kit: 2 x order no. 17008
17009 Mobile Phase B 800 ml
17011 Standard Solution 5 ml
17012 IS Internal Standard 5 ml
17013 Urine Calibrator, lyophil. 5 x 5 ml
17020 Sample preparation vials 100 pcs.
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
17030 Analytical Column with test chromatogram 1 pce.
17032 Guard Column Holder incl. 1 Guard Column 1 pce.
17033 Guard Columns 5 pcs.
 
  Accessories:  
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
17080 Urine Control, lyophil., Level I 10 x 5 ml
17081 Urine Control, lyophil., Level II 10 x 5 ml
17082 Urine Control, lyophil., Level I, II 2 x 5 x 5 ml


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