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Hydroxyproline in Urine
Complete Kit, Order No. 16000

 

Osteoporosis is a metabolic bone disease that is characterized by an abnormally low bone density and therefore is associated with an increased risk of fracture. The bone degradation goes along with an increased formation of the amino acid hydroxyproline, which is released into blood circulation and is excreted with urine.

The test kit of RECIPE guarantees an accurate hydroxyproline measurement. By a special sample preparation, hydroxyproline is turned into a derivative and can be quantified without interferences from other amino acids.

 
  • Reliable sample preparation
    (specific derivatisation of the analyte)

  • Chromatography without interferent substances

  • Injection interval: 8 min


Test Data

  

HPLC Parameters
Linearity: 5 - 170 mg/l
Recovery: 90 - 100 %
Lower detection limit: 3 mg/l
Lower determination limit: 5 mg/l
Intraassay precision: 3.5 %
Interassay precision: 4.5 %
 
Pump: binary gradient pump,
flow rates: 1.2, 1.4 ml/min
Injection volume: 20 µl
Injection interval: 8 min
UV/VIS detector: 471 nm
HPLC-Thermostat: 60°C

 

Sample Preparation

16000_flow

Chromatogramms
ClinChek® Urine Control, Level I ClinCheck® Urine Control, Level II
16000_chrom1   16000_chrom2
Hydroxyproline: 3.20 min, IS: 5.10 min   Hydroxyproline: 3.20 min, IS: 5.10 min


Hydroxyproline in Urine
Complete Kit, Order No. 16000

Ordering Information

Order No. Description Quantity
16000  ClinRep® Complete Kit for Hydroxyproline in Urine for 100 assays 1 pce. 
 
  Kit Components (order no. 16000):  
16008 Mobile Phase A 1000 ml
16009 Mobile Phase B 150 ml
16011 Standard Solution 5 ml
16012 IS Internal Standard 10 ml
16013 Urine Calibrator, lyophil. 5 x 8 ml
16020 Hydrolysis Tubes with caps 100 pcs.
16021 ph - Test Stripes 100 pcs.
16022 Reagent A 30 ml
16023 Reagent B 80 ml
16024 Reagent C 80 ml
16025 Reagent D 50 mg
within the kit: 2 x order no. 16025
16026 Reagent E 20 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
16030 Analytical Column with test chromatogram 1 pce.
 
  Accessories:  
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
8820 Urine Control, lyophil., Level I 10 x 8 ml
8821 Urine Control, lyophil., Level II 10 x 8 ml
8822 Urine Control, lyophil., Level I, II 2 x 5 x 8 ml


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