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Hemoglobin Variants and ß-Thalassemia
Screening in Whole Blood
Complete Kit, Order No. 13100

 

Defects in genes, which are responsible for the synthesis of the hemoglobin molecule, result in a group of hereditary diseases that are termed as "hemoglobinopathies". Depending on the molecular defect, hemoglobinopathies may be classified in "structural hemoglobin variants" and in "thalassemias".

This test kit allows for a reliable separation and identification of the common and clinically significant hemoglobin variants. For ß-thalassemia screening the kit enables an exact quantification of HbF and HbA2. A ready-to-use test solution (contains: HbA1c, HbF, HbA, HbE, HbA2, HbD, HbS, HbC), a calibrator hemolysate (contains: HbF, HbA2) as well control hemolysates in two levels of concentration (each contains: HbF, HbA2, HbS) are available for quality assurance of the analytical results.

  
  • Reliable quantification of
    HbA2 and HbF


  • Safe identification of
    HbS, HbC, HbE and HbD


Test Data

  

HPLC Parameters
Intraassay precision: 1.0 - 3.3 % (HbF)
  0.6 - 3.6 % (HbA2)
Interassay precision: 2.1 - 2.8 % (HbF)
  0.7 - 2.4 (HbA2)
   
Dependance on total hemoglobin:
The HbF and HbA2 values are independent on the amount of total hemoglobin in a range from 6 - 24 g/dl total hemoglobin.
 
Pump: Binary gradient pump
Flow rate: 1.2 ml/min
Injection volume: 20 µl
Injection interval: 8 min
UV/VIS-Detector: 415 nm
HPLC Thermostat: 23 °C


Sample Preparation

SP Hb-Variants

Chromatograms
ClinChek® Calibrator-Hemolysate (HbF/A2) ClinCheck® Control-Hemolysate (HbF/A2/S), Level I
 
HbA1c: 1.59 min, HbF: 2.03 min, HbA0: 3.74 min, HbA2: 4.71 min   HbA1c: 1.58 min, HbF: 2.01 min, HbA0: 3.72 min, HbA2: 4.71, HbS: 5.52min

 

Hemoglobin Variants and ß-Thalassemia
Screening in Whole Blood
Complete Kit, Order No. 13100

Ordering Information

Order No. Description Quantity
13100  ClinRep® Complete Kit f. Hemoglobin Variants a. ß-Thalassemia Screening i. Whole Blood for 500 assays 1 pce. 
 
  Components of the Complete Kit (order no. 13100):  
13101 ClinRep® Reagent Kit 1 pce.
13113 Calibrator-Hemolysate HbF/A2, ready to use 5 x 3 ml
13130 Analytical Column with test chromatogram 1 pce.
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Components of the Reagent Kit (order no. 13101):  
13107 Buffer 1 2500 ml
13108 Buffer 2 2500 ml
13109 Washing Solution 2000 ml
13121 Hemolysis Reagent 500 ml
     
  Optional Components:  
13114 Test Solution (HbA1c/F/A/E/A2/D/S/C), ready to use 5 x 3 ml
 
  Accessories:  
11024 Sample vials 1.5 ml without cap 1000 pcs.
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
13182 Control-Hemolysate HbF/A2, Level I, II, ready for use 2 x 5 x 3 ml
13183 Control-Hemolysate HbF/A2/S, Level I, ready for use 5 x 3 ml

 

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