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Metanephrines in Urine | Automated Sample Preparation with ClinLab® APS3000 Complete Kit,
Order No. 4300

 

As an alternative to the determination of the urinary metanephrines with manual sample preparation (see ClinRep® complete kit with order no. 4000), RECIPE offers appropriate ClinRep® complete kits for use with automatic pipetting systems from Gilson® (see order nos. 4100-4200) and RECIPE ClinLab® APS3000.

There, the sample preparation and -injection is performed fully automated. In advance, the samples must be hydrolysed manually in order to ensure a quantitative release of sulfateester- and glucuronide bound urinary metanephrines (manual sample pretreatment).

  
  • Test kits for 100 or 500 assays available


  • Robust Chromatography


  • Injection interval: 17 min


Test Data

  

HPLC Parameters
Linearity: 7 - 10000 µg/l
  (per analyte)
Recovery: 98 %
Lower detection limit: 4 µg/l
  (per analyte)
Lower determination limit: 7 µg/l
  (per analyte)
Intraassay precision: 1.0 % (NM)
  1.5 % (M)
  1.8 % (MT)
Interassay precision: 1.0 % (NM)
  2.6 % (M)
  2.9 % (MT)
   
NM: Normetanephrine, M: Metanephrine, MT: 3-Methoxytyramine
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 20 µl
Injection interval: 17 min
EC-Detector: potential: 650 mV,
sensitivity: 50 nA (settings
for RECIPE detector EC3000
HPLC-Thermostat: 30°C

 

Sample Preparation

4300_flow

 

Chromatograms
ClinChek® Urine Control, Level I ClinCheck® Urine Control, Level II
4300_chrom1   4300_chrom2
Normetanephrine: 5.26 min, Metanephrine: 6.47 min,
IS: 9.43 min, 3-Methoxytyramine: 15.03 min		   Normetanephrine: 5.25 min, Metanephrine: 6.48 min,
IS: 9.42 min, 3-Methoxytyramine: 15.03 min


Metanephrines in Urine | Automated Sample Preparation with ClinLab® APS3000 Complete Kit,
Order No. 4300

Ordering Information

Order No. Description Quantity
4300   ClinRep® Complete Kit for Metanephrines in Urine(Automated Sample Preparation with ClinLab® APS3000) for 100 assays 1 pce.  
 
  Kit Components (order no. 4300):  
4010 Mobile Phase 1000 ml
4011 Standard Solution 10 ml
4012 IS Internal Standard 3 ml
4013 Urine Calibrator, lyophil. 5 x 8 ml
4320 Sample Preparation Columns 50 pcs.
(within the kit: 2 x order no. 4320)
4022 D Diluting Solution 400 ml
4323 W Washin Solution 500 ml
4124 E Eluting Reagent 250 ml
4125 S Stabilizing Reagent 80 ml
FK6401 Sample vials, clear glass, 4ml 100 pcs.
(withing the kit 2 x order no. FK6401)
  Manual 1 pce.
  Quick Reference 1 pce.
 
4400 ClinRep® Complete Kit for Metanephrines in Urine 1 pce.
  (Automated Sample Preparation with ClinLab® APS3000)  
  for 500 assays  
 
  Kit Components (order no. 4400):  
4010 Mobile Phase 1000 ml
(within the kit: 5 x order no. 4010)
4011 Standard Solution 10 ml (within the kit: 5 x order no. 4011)
4012 IS Internal Standard 3 ml
(within the kit: 5 x order no. 4012)
4013 Urine Calibrator, lyophil. 5 x 8 ml (within the kit: 5 x order no. 4013)
4320 Sample Preparation Columns 50 pcs. (within the kit: 10 x order no. 4320)
4022 D Diluting Solution 400 ml (within the kit: 5 x order no. 4022)
4323 W Washin Solution 500 ml (within the kit: 5 x order no. 4323)
4124 E Eluting Reagent 250 ml (within the kit: 5 x order no. 4124)
4125 S Stabilizing Reagent 80 ml (within the kit: 5 x order no. 4125)
FK6401 Sample vials, clear glass, 4ml 100 pcs. (withing the kit: 10 x order no. FK6401)
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
4030 Analytical Column with test chromatogram 1 pce.
 
  Accessories:  
FK6300 Reagent tube (100 x 16 mm) with screw cap
and o-ring, PP, autoclavable up to 121 °C		 50 pcs.
 
  ClinChek® Controls:  
8820 Urine Control, lyophil., Level I 10 x 8 ml
8821 Urine Control, lyophil., Level II 10 x 8 ml
8822 Urine Control, lyophil., Level I, II 2 x 5 x 8 ml