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VMA, HVA and 5-HIAA in Urine | Automated Sample Preparation with Gilson® ASPEC™ XL Complete Kit, Order No. 3100 and 3200

 

As an alternative to the determination of VMA, HVA and 5-HIAA with manual sample preparation (see ClinRep® complete kit with order no. 3000), RECIPE offers appropriate ClinRep® complete kits for the use with automatic pipetting systems from RECIPE (see order nos. 3300-3400) and Gilson® (see below). There, the sample preparation and -injection is performed fully automatically.

The ClinRep® complete kit for the determination of VMA, HVA and 5-HIAA with the automatic pipetting system Gilson® Aspec™ XL contains all reagents, that are required for analysis, including instrument adapted sample preparation columns.

 
  • Automated sample preparation

  • Robust chromatography

  • Testkits available for 100 and 500 assays


Test Data

  

HPLC Parameters
Linearity: VMA: 0.6 - 100 mg/l
  HVA: 0.3 - 100 mg/l
  5-HIAA: 0.2 - 50 mg/l
Recovery: 75 - 90 %
Lower detection limit: VMA: 0.2 mg/l
  HVA: 0.1 mg/l
  5-HIAA: 0.1 mg/l
Lower determination limit: VMA: 0.6 mg/l
  HVA: 0.3 mg/l
  5-HIAA: 0.2 mg/l
Intraassay precision: VMA: 1.4 %
  HVA: 1.9 %
  5-HIAA: 2.9 %
Interassay precision: VMA: 1.8 %
  HVA: 2.6 %
  5-HIAA: 2.6 %
 
Pump: isocratic pump,
flow rate: 0.9 ml/min
Injection volume: 20 µl
Injection interval: 15 min
EC-detector: potential: 800 mV,
sensitivity: 50 nA (settings
for RECIPE detector EC3000
(see pages 150-151)
HPLC-Thermostat: 30°C

 

Sample Preparation

 

3100_flow

 

Chromatograms
ClinChek® Urine Control, Level I ClinCheck® Urine Control, Level II
3100_chrom1   3100_chrom2
VMA: 3.67 min, IS: 5.50 min, HVA: 7.99 min,
5-HIAA: 11.80 min
   VMA: 3.62 min, IS: 5.46 min, HVA: 7.96 min,
5-HIAA: 11.77 min

 

VMA, HVA and 5-HIAA in Urine | Automated Sample Preparation with Gilson® ASPEC™ XL Complete Kit, Order No. 3100 and 3200

Ordering Information

Order No. Description Quantity
3100   ClinRep® Complete Kit for VMA, HVA and 5-HIAA in Urine(automated sample preparation with Gilson® Aspec™ XL) for 100 assays 1 pce.  
 
  Kit Components (order no. 3100):  
3010 Mobile Phase 1000 ml
3011 Standard Solution, lyophil. 5 x 1 ml
3012 IS Internal Standard 100 ml
3013 Urine Calibrator, lyophil. 5 x 5 ml
3120 Sample Preparation Columns 50 pcs.
within the kit: 2 x order no. 3120
3021 A Ammonia Solution 100 ml
3022 B Boric Acid Solution 300 ml
within the kit: 2 x order no. 3022
3023 E Eluting Reagent 100 ml
within the kit: 2 x order no. 3023
  Manual 1 pce.
  Quick Reference 1 pce.
 
3200   ClinRep® Complete Kit for VMA, HVA and 5-HIAA in Urine(automated sample preparation with Gilson® Aspec™ XL) for 500 assays 1 pce.  
     
  Kit Components (order no. 3200):  
3010 Mobile Phase 1000 ml
within the kit: 5 x order no. 3010
3011 Standard Solution, lyophil. 5 x 1 ml
within the kit: 5 x order no. 3011
3012 IS Internal Standard 100 ml
within the kit: 5 x order no. 3012
3013 Urine Calibrator, lyophil. 5 x 5 ml
within the kit: 5 x order no. 3013
3120 Sample Preparation Columns 50 pcs.
within the kit: 10 x order no. 3120
3021 A Ammonia Solution 100 ml
within the kit: 5 x order no. 3120
3022 B Boric Acid Solution 300 ml
within the kit: 10 x order no. 3022
3023 E Eluting Reagent 100 ml
within the kit: 10 x order no. 3023
  Manual 1 pce.
  Quick Reference 1 pce.
     
  Start Accessories:  
3030 Analytical Column with test chromatogram 1 pce.
 
  Refill Service:  
3031 Analytical Column with test chromatogram 1 pce.
  (only if you send back your original column)  
     
  ClinChek® Controls:  
8820 Urine Control, lyophil., Level I 10 x 8 ml
8821 Urine Control, lyophil., Level II 10 x 8 ml
8822 Urine Control, lyophil., Level I, II 2 x 5 x 8 ml