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Catecholamines in Urine | On-Line Analysis
Complete Kit, Order No. 2100

 

This test is designed as a fast (injection interval: 9.5 min) and fully automated method for the analysis of urinary catecholamines. A manual sample preparation is not required, but is performed „on-line“ within the HPLC system.

Alternatively to this method with manual sample preparation, RECIPE offers ClinRep® complete kits for the use with automatic pipetting systems from RECIPE (order nos. 2400-2500) and GILSON (order nos. 2200-2300).

  
  • Fully automatic method with on-line sample preparation

  • Robust chromatography with fluorescence detection

  • Injection interval: 20 min


Test Data

  

HPLC Parameters
Linearity: 3 - 1500 µg/l
  (per analyte)
Recovery: 95 - 100 %
Lower detection limit: 1 µg/l
  (per analyte)
Lower determination limit: 3 µg/l
  (per analyte)
Intraassay precision: 3.0 % (norepinephr.)
  4.5 % (epinephrine)
  0.8 % (dopamine)
Interassay precision: 3.6 % (norepinephr.)
  6.4 % (epinephrine)
  2.4 % (dopamine)
 
Pump 1:
(SPE buffer)		 isocratic pump,
flow rates: 0.5, 2.0 ml/min
Pump 2:
(Mobile Phase)
 isocratic pump,
flow rate: 1.2 ml/min
Swithcing valves: 6-port / 3-channel switching
valve (order no. FK1100)
Injection volume: 50 µl
Injection interval: 20 min
Fluorescence detector: 275 nm (exc.), 330 nm (em.)
HPLC-Thermostat: 30°C
   
botton_Recipe System Configuration

 

Sample Preparation

2100_flow

 

Chromatograms
ClinChek® Urine Control, Level I (+ 20 µl IS/ml urine) ClinCheck® Urine Control, Level II (+20 µl IS/ml urine)
2100_chrom1   2100_chrom2
Norepinephrine: 9.12 min, Epinephrine: 10.52 min,
IS: 12.85 min, Dopamine: 16.11 min		   Norepinephrine: 9.11 min, Epinephrine: 10.51 min,
IS: 12.84 min, Dopamine: 16.10 min


Catecholamines in Urine | On-Line Analysis
Complete Kit, Order No. 2100

Ordering Information

Order No. Description Quantity
2100  ClinRep® Complete Kit for Catecholamines in Urine
for 500 assays		 1 pce. 
 
  Kit Components (order no. 2100):  
2109 SPE - Buffer 2500 ml
within the kit: 4 x order no. 2109
2110 Mobile Phase 2500 ml
within the kit: 6 x order no. 2110
2011 Standard Solution 10 ml
2113 Urine Calibrator (FD), lyophil. 5 x 5 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Optional Components:  
2012 IS Internal Standard 3 ml
 
  Start Accessories:  
1232 Guard Column Holder 1 pce.
1233 Guard Column 4 pcs.
2130 Analytical Column with test chromatogram 1 pce.
2131 SPE - Column 1 pce.
FK5810 PEEK capillary connector 1 pce.
FK5820 Stainless steel profilter holder 1 pce.
FK7400 Inline-Filter (stainless steel sieve, free of dead volume) 1 pce.
 
  Accessories:  
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
FK7330 Endfittings 2 pcs.
FK7340 Sealings and sieves 4 pcs. each
FK7350 Endstoppers 2 pcs.
 
  ClinChek® Controls:  
2180 Urine Control (FD), lyophil., Level I 10 x 5 ml
2181 Urine Control (FD), lyophil., Level II 10 x 5 ml
2182 Urine Control (FD), lyophil., Level I, II 2 x 5 x 5 ml