PCR Quantification Standards
for Highest Accuracy

Cat.-No. 52-0101           Legionella pneumophila
Cat.-No. 52-0112           Mycoplasma orale
Cat.-No. 52-0119           Mycoplasma pneumoniae
Cat.-No. 52-0116           Acholeplasma laidlawii
Cat.-No. 52-0117           Mycoplasma fermentans
Cat.-No. 52-0130           Mycoplasma hyorhinis
Cat.-No. 52-0440           Chlamydia trachomatis

Applications
The DNA standards can be used as a general control to confirm PCR system per formance using a qPCR instrument. Calibration curves are highly reproducible and allow the generation of highly specific, sensitive and reproducible data. The concentration of the Quantification Standard was determined photometrically and by qPCR. The DNA concentration was adjusted by dilution with DNA stabilizing buffer. Genomic DNA is used as a control template when DNA amplification tests by conventional PCR are performed (e.g. as contamination control in cell culturing). With the qPCR technology, this titrated DNA standard with a defined concentration of genome copies is used as calibrator to generate standard curves. Therefore the provided solution is diluted in a 10x dilution series with DNA-free water and used as a PCR sample. With the generated fluorescent data, the software provided with the qPCR instrument calculates a standard curve, which can be used to determine the DNA concentration of unknown samples.

Extraction
The quantification standard is genomic DNA extracted from low passage and de fined microorganisms. The extraction of the DNA is done by phenol/chloroform extraction with ethanol precipitation and column absorption methods. These DNA-Standards allow for the performance of a control reaction for PCR using qPCR instrumentation and to create a standard curve for quantification experiments, i.e. with Venor®Mp Mycoplasma pneumoniae Diagnostic Kit for qPCR. The DNA standards can be used as a general control to confirm PCR system performance using a qPCR instrument or for the validation of detection limits in respect to conventional PCR tests.

Contents
1 vial (green cap) of Standard DNA, 100 μl, contains approx. 106 genomes/μl.
3 vials (white cap) with 1 ml of Tris-HCl buffer, 10mM, pH 8.5.

 

 

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