Onar® Pertussis:
Bordetella pertussis / parapertussis for qPCR

Type 1                            Type 2                              Pack Size
Cat.-No. 25-2025              Cat.-No. 25-3025                 25 Tests
Cat.-No. 25-2100              Cat.-No. 25-3100                100 Tests
Cat.-No. 25-2250              Cat.-No. 25-3100                250 Tests

Description
The Onar®Pertussis system is an in vitro test for the quantitative and rapid PCR diagnosis of Bordetella pertussis and B. parapertussis in clinical samples. Onar®Pertussis will be CE-marked and approved for in vitro clinical diagnostics.

Versatility Onar®Pertussis is compatible with the LightCycler®, Rotor-GeneTM, Opticon®, ABI Prism®, SmartCycler®, Mx3000p® and other devices.   Sensitive Detection of less than 10 pathogen particles per sample volume.   Specific The primers used in the Onar®Pertussis kit are specific for Bordetella pertussis and B. parapertussis.   Reliable and rapid The kit contains a positive control and a homologous internal control for optimal PCR quality check.   Convenient The convenient master mix format only requires a few pipetting steps.

 

Background
Pertussis, or whooping cough, was a major cause of infant and childhood mor-tality until the introduction of vaccine programs in the 1940s. In the last two decades pertussis has undergone a resurgence. An increase in incidence has been reported for several countries, whereas an obvious shift in the prevalence of infants and children to adolescents and adults can be observed. Because the symptoms in older and/or previously immunized persons are often atypical, pertussis may be difficult to differentiate from other respiratory tract infections. A reliable and fast in vitro diagnostic system as the Onar®Pertussis can help preventing further transmission of this highly communicable respiratory disease.

Kit Components

• primer, probe and nucleotides at optimized concentrations
• reaction buffer optimized for MB Taq DNA Polymerase
• positive control for easy result verification
• internal control to ensure accurate testing
• instruction manual

Only purified sample DNA and Taq polymerase need to be added.
Note: Onar®Pertussis is validated and recommended for use with our reliable hot-start MB Taq DNA Polymerase (Cat.-No. 53-0200).

Test Principle
The Onar®Pertussis test system is an in vitro test for the quantitative diagnosis of Bordetella pertussis and/or B. parapertussis in clinical samples. The supplied primer and probe are specific for the dermonecrotic toxin gene (dnt gene) of the genomes of both species. The target probe emits fluorescent light at ~520 nm. To provide assurance that clinical specimens are free of PCR inhibitors, the kit includes an internal amplification control (IAC), that is detected by another probe emitting light at ~610 nm (Type 1) or ~560 nm (Type 2). The IAC is constructed as a homologous control containing primer binding sites identical to the target but a unique internal sequence.

 

Instrument Compatibility

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