Onar® Ct:
Chlamydia trachomatis for qPCR

Type 1                            Type 2                              Pack Size
Cat.-No. 24-2025              Cat.-No. 24-3025                 25 Tests
Cat.-No. 24-2100              Cat.-No. 24-3100                100 Tests
Cat.-No. 24-2250              Cat.-No. 24-3100                250 Tests

Description
The Onar®Ct is an in vitro test for quantitative and rapid PCR-based detec-tion of Chlamydia trachomatis (Ct) DNA in clinical samples. These samples may be extracted from infected or routinely screened patients. Patient specimens like urine are used as starting material for the DNA extraction.

Sensitive Quantitative detection of less than 0.9 IFU/μl. DNA standards are separately available for reliable quantification.   Specific Onar®Ct is designed to amplify the chro-mosomal gene momp (major outer-membrane protein). The momp gene is a single copy gene which is suitable for quantification. Furthermore the test detects the new variant Chlamydia tra-chomatis (nvCT) representing a deletion in the cryptic plasmid, (MØller JK et al., 2008, Alexander S. and Ison C., 2008).   Reliable and rapid The kit contains a positive control DNA and a specific internal control probe for optional PCR quality check. Results are obtained within 2-3 hrs.   Convenient Detailed protocols for dif-ferent qPCR cyclers and sample preparation methods includ-ing urine pools allow an instant and simple application of the kit.

 

Background
Chlamydia trachomatis is an intra­cellular, gram-negative bacterium. Infection of the lower genital tract is one of the most prevalent sexually trans­mitted diseases (STD) in the world. Chlamydia trachomatis infection may cause urethritis, cervicitis and pelvic inflammatory disease (PID) in women. Detecting chlamydial genital infection and preventing transmission and spread of this infection to the upper reproductive tract are a challenge for both clinician and laboratory workers. Nucleic acid amplification techniques are used with increasing frequency to diagnose chlamydial infections.

Kit Components

• primer, probe and nucleotides at optimized concentrations
• buffer optimized for MB Taq DNA Polymerase
• positive control for easy result verification
• internal control to prevent false negative results by PCR inhibition
• instruction manual

Only purified sample DNA and Taq polymerase need to be added.
Note: Onar®Ct is validated and recommended for use with our reliable hot-start MB Taq DNA Polymerase (Cat.-No. 53-0200) and our DNA Ex­traction Kit (Cat.-No. 56-1100).

Test Principle
Onar®Ct is a test for quantitative di­agnostics of clinical samples. The test can basically be used with all relevant PCR machines. The supplied primer, probe and the positive control are spe­cific for a segment of the momp gene of the Chlamydia trachomatis genome.  The target probe emits fluorescent light at ~520 nm. To rule out the presence of PCR inhibitors in the individual pa­tient specimens, an internal control is included in the kit. The internal control is detected by another probe emitting light at ~610 nm (Type 1) or ~560 nm (Type 2).

 

Instrument Compatibility

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