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Venor® Mp:
Mycoplasma Pneumoniae for qPCR
Type 1 Type 2 Pack Size
Cat.-No. 20-2025 Cat.-No. 20-3025 25 Tests
Cat.-No. 20-2100 Cat.-No. 20-3100 100 Tests
Cat.-No. 20-2250 Cat.-No. 20-3250 250 Tests
Description
The Venor®Mp-QP system is an in vitro test for the quantitative diagnosis of Mycoplasma pneumoniae in clinical samples. Venor®Mp-QP is CE-marked and approved for in vitro clinical diagnostics. The test system has undergone extensive internal validation, as well as external clinical evaluations.
Versatility
Venor®Mp-QP is compatible with the LightCycler®, Rotor-GeneTM, Opticon®, ABI 7500®, SmartCycler®, Mx3000p® and other devices.
Sensitive
Detection of less than 10 Mycoplasma pneumoniae particles per sample volume.
Specific
The primers used in the Venor®Mp-QP kit were selected to exclusively amplify Mycoplasma pneumoniae subtype I and II DNA encoding the P1 adherent protein.
Reliable
The kit contains internal control DNA as an optional PCR quality check and positive control DNA to optimize reproducibility and minimize operator dependent variability of results.
Excellent detection range
The detection range of the assay is 5 to 107 copies of Mycoplasma pneumoniae genome.
Background
Please refer to Venor®Mp for background information on M. pneumoniae. Venor®Mp-QP incorporates the highly-sensitive qPCR technology and overcomes all drawbacks of other techniques with its unique features.
Kit Components
- primer, probe and nucleotides at optimized concentrations
- reaction buffer optimized for MB Taq DNA Polymerase
- positive control for easy result verification
- internal control to prevent false negative results
- instruction manual
Only purified DNA and Taq polymerase need to be added.
Note: Venor®Mp-QP is validated and recommen-ded for use with our reliable hot-start MB Taq DNA Polymerase (Cat.-No. 53-0200).
For calibration we recommend our DNA Quantification Standards (Mycoplasma pneumoniae, Cat.-No. 52-0119).
Test Principle
The Venor®Mp-QP is a test system for the qualitative and quantitative diagnosis of in clinical samples. The test is based on the polymerase chain reaction and allows for rapid diagnosis of infections. The supplied primer and the probe are specific for a segment of the P1 operon region of the mycoplasma genome. The target probe emits fluorescent light at 520 nm. To rule out the presence of PCR inhibitors in the individual patient specimens, an internal control is included in the kit. The internal control is detected by another probe emitting light at ~610 nm (Type 1) or ~560 nm (Type 2).
The optional M. pneumoniae Quantification Standard (Cat.-No. 52-0119) provides DNA for the quantification of the DNA load in the sample.
DNA extracts of nasopharyngeal swabs, nasal and pharyngeal secretions, sputum, provoked sputum, bronchial lavage, tissue and infected cells or cutures can be used as test materials.
Instrument Compatibility
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