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iris technologies international gmbh

Venor® Mp:
Mycoplasma Pneumoniae for conventional PCR

Cat.-No. 20-1025      25 Tests
Cat.-No. 20-1100      100 Tests
Cat.-No. 20-1250      250 Tests

Description
The Venor®Mp system is an in vitro test for the qualitative diagnosis of Mycoplasma pneumoniae in clinical samples. The test is based on the polymerase chain reaction, and thus providing highly sensitive and 100 % specific rapid diagnosis. Venor®Mp is CE-marked and approved for in vitro clinical diagnostics. The test system has undergone extensive internal validation, as well as external clinical evaluations.

Sensitive
Detection of approx. 5 Mycoplasma pneumoniae particles per sample volume.

Specific
The primers used in the Venor®Mp kit were selected to exclusively amplify M. pneumoniae subtype I and II DNA encoding the P1 adherent protein.

Reliable
The kit contains internal control DNA as an optional PCR quality check and positive control DNA to optimize reproducibility and minimize operator dependent variability of results. Convenient The convenient master mix format only requires a few pipetting steps. Broad sample diversity Templates for PCR analysis are prepared by DNA extraction from throat swabs, sputum, bronchoalveolar lavage, pleural fluid, or lung biopsies.

Convenient
The convenient master mix format only requires a few pipetting steps.

Broad sample diversity
Templates for PCR analysis are prepared by DNA extraction from throat swabs, sputum, bronchoalveolar lavage, pleural fluid, or lung biopsies.

Background
Mycoplasma pneumoniae is a continual source of upper respiratory infections throughout the year and accounts for about 20 % of community acquired pneumonias. The spread increases within closed populations to 80 %. The incidence of infection is greatest in older children, adolescents, and young adults. The initial presenting symptoms of M. pneumoniae infection after a 2-3 week incubation period may include cough, fever, malaise, chills, sore throat, and headache. Asymptomatic infec tions account for about 20 % of cases. Mycoplasma pneumoniae infections tend to be more chronic and indis tingishable from viral causes and other atypical pathogens on the basis of clini cal symptoms alone. Thus, in many clini cal settings, accurate and rapid diagno sis is important for an efficient patient management. Diagnosis of acute infec tions is usually based on culture and serology, known to be difficult by means of insensitivity, delayed detection, and poor specificity. Venor®Mp Mycoplasma pneumoniae diagnostic kit incorporates the highly-sensitive PCR technology and overcomes all drawbacks of these other techniques with its unique features.

Kit Components

• primer set and nucleotides at optimized concentrations
• 10x reaction buffer optimized for MB Taq DNA Polymerase
• positive control for easy result verification
• internal control to ensure accurate testing
• instruction manual

Only purified DNA and Taq polymerase needs to be added.
Note: Venor®Mp is validated and recommended for use with our reliable hot-start MB Taq DNA Polymerase (Cat.-No. 53-0200).

Test Principle
The Venor®Mp diagnostic kit uses specific primers to amplify a 207 bp nucleotide sequence within the highly conserved and multiple copy P1 cytadhesin gene. Various stand ard DNA preparation methods can be used for extraction of microbial DNA from respiratory samples followed by Venor®Mp PCR amplification. The amplicon is detected by standard agarose gel electrophoresis. To rule out the presence of PCR inhibitors in the patient specimen, an internal control is included in the kit. When there is no target DNA in the sample, a positive result from the internal control demonstrates that the PCR worked.

 

Fig.
Amplified PCR products are visualized by standard gel electrophoresis. A sample contain ing Mycoplasma pneumoniae will produce a distinct 207 bp band. The internal control should be present in every reaction and will produce a 263 bp band. The internal control band is less visible with samples of strong infections.

 

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