DNA Remover™ :
Decontamination Spray for the Removal of DNA

Cat.-No. 15-2025              Spray bottle (250 ml)
Cat.-No. 15-2200              Refill bottles (4 x 500 ml)

To prevent contaminations divide the work area and thoroughly clean them with DNA decontamination solutions.

Description
DNA Remover™ is a novel, non-alkaline and non-carcinogenic cleansing solution that is highly active against DNA and RNA contaminations. DNA Remover™ is intended for use at PCR workstations. The solution contains a surfactant and a DNA destroying agent.

Highly effective DNA Remover™ is effective against amplicon, plasmid, or genomic DNA and RNA from any surface. Convenient and ready-to-use DNA Remover™ is ready-to-use for eliminating DNA and RNA from any surface. Fast and easy decontamination The use of DNA Remover™ both before and after PCR analysis is fast, easy and ideal to maintain a clean work area and thereby saves time and expenses. Stable DNA Remover™ is stable for several years and heat resistant. Non-alkaline and non-carcinogenic

Background
Contaminant DNA is especially problematic in the highly sensitive PCR technique, as its removal has proven to be no trivial matter. Originating from aerosolized fragments, contaminant DNA can lead to cross contamination between samples thus resulting in inaccurate data or PCR artifacts. Even a single DNA molecule can be detected in the amplification process leading to widespread problems throughout the testing procedure.
DNA RemoverTM is ready-to-use for eliminating DNA from any surface. By following the decontamination instructions below, DNA is completely destroyed and removed. DNA RemoverTM should be stored at room temperature. Storage for 2 weeks at 65 °C does not reduce the quality of the product.

Protection and Precautions
Eye contact and prolonged contact of DNA Remover TM with skin may cause irritation. Therefore, safety glasses and disposable gloves should be worn while handling the reagent. DNA Remover TM should be applied on glass, ceramic, plastic, rubber, steel and precious met­al. DNA Remover TM can not be used for the cleaning of light or non-ferrous metals. To avoid damage or discoloration, it is recommended to spot test sensitive surfaces prior to use.
For the decontamination of smooth, non-porous surfaces spray DNA RemoverTM directly on the surface and wipe thoroughly with a paper towel. Rinse immediately and thoroughly with clean water and dry with a clean paper towel.

Decontamination of Lab Apparatus
For the treatment of laboratory devices apply to a paper towel and wipe all ex­posed surfaces of the apparatus thoroughly. Rinse immediately with clean water and dry with a clean paper towel. To clean small parts, briefly soak them in DNA RemoverTM and rinse with clean water and dry.

Decontamination of Pipettors
Contamination of pipettors may even occur using filtered tips. For the decontamination follow the manufacturer´s instructions and remove the shaft from the pipettor. Remove seals and gaskets from the shaft. Soak the shaft for one minute in DNA RemoverTM. Rinse im­mediately and thoroughly with clean water, dry and reassemble.

Suggestions for Maintaining a PCR Lab
Sample preparation, PCR set-up, and post PCR manipulation and analysis should be performed in separate areas, preferably in separate rooms if feasible.
Use fresh, sterile disposables for every step of the extraction process to minimize the chance of cross-contamination. If this is not possible, all items to be reused should be cleaned with DNA RemoverTM prior to reuse.
Pipettors should be designated for PCR use only. A separate set of pipettors for sample preparation, PCR setup, and analysis is highly recommended. If not possible, pipettors can be cleaned regularly with DNA RemoverTM and ex­posed to U.V. irradiation at least once a week.
All areas used in PCR analysis should be cleaned thoroughly with DNA RemoverTM before and after use.

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